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The wide range of StageTips available enables you to select according to the type of purification required and the amount of sample to be purified. Approximate loading capacities are specified for each product. StageTips using GE Loader pipette tips are ideal for depositing cleaned sample directly into the emitter with minimal sample loss when performing off-line nano ES MS. StageTips are disposable polypropylene pipette tips containing a microcolumn insert and fitting onto standard pipettes and most commercial robotic systems. The PTFE-based microcolumn insert supports densely packed particles of chromatography material. Each pack of StageTips is supplied with a 2.5 mL Eppendorf Combitip to facilitate loading, elution and washing of the microcolumn. Instructions for Use are supplied and can also be downloaded below. (Instructions cover the use of C8, C18 and SCX chromatographic material when preparing samples for nanoelectrospray, LC MS and MALDI MS.)
For further information please login to download the file below.
Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Juri Rappsilber, Matthias Mann, Yasushi Ishihama, Nat Protoc. 2007;2(8):1896-906 Modular Stop and Go Extraction Tips with Stacked Disks for Parallel and Multidimensional Peptide Fractionation in Proteomics. Yasushi Ishihama, Juri Rappsilber and Matthias Mann, Journal of Proteome Research 2006, 5, 988-994 Stop and Go Extraction Tips for Matrix-Assisted Laser Desorption/Ionization, Nanoelectrospray, and LC/MS Sample Pretreatment in Proteomics. Juri Rappsilber, Yasushi Ishihama and Matthias Mann, Anal. Chem. 2003, 75, 663-670 |
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