Fast, fail-safe purification of peptide and protein samples

Highest quality results are critically dependent on optimal sample preparation. Following on from a concept first decribed by Rappsilber et al (Anal. Chem. 2003, 75, 663-670), Proxeon has developed the StageTip range -  ready to use, prepacked microcolumns for purification. Applications include desalting, pre-concentration, and separation (by step elution) of samples for MALDI, LC-MS and nano ES-MS.

  • Ensure cost-efficiency

    • save time and eliminate errors associated with alternative solutions

  • Choose from C8, C18 or SCX (cation exchange) chromatographic media

    • preselected to ensure minimal unspecific and irreversible binding

  • Follow a simple, effective generic protocol

    • developed to ensure efficient binding  in a single pass

    • load, wash and elute at >300µl/min using low back pressure
  • Achieve excellent sensitivity in the low femtomole to high attomole range
  • Ensure high reproducibility
    • no tip variation, reliable protocols and superb ease of use
  • Increase throughput
    • ready-made, disposable tips are ideal for lab robots and multi-channel pipettes

Latest publication: Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips. Rappsilber, Mann and Ishihama, Nat Protoc. 2007;2(8):1896-906