Nano-LC- EASY-nLC 1000

Frequently asked questions

Q: Where can I find the latest Xcalibur driver for the EASY-nLC system?

The latest driver is included in LC devices, which can be ordered. Contact your local sales office for more info.

 

Q: Where can I find the latest version of instrument software for the EASY-nLC?

The latest driver can be found on the EASY-nLC User Zone - see below.

Q: What is Load Speed Protection (LSP)?

Load speed protection is designed to ensure that each sample is loaded with identical flow rates in order to maximize sample to sample reproducibility for quantitation experiments. LSP is only active during sample loading and for proper use of the LSP feature it is required to enter both a loading flow and a loading max pressure limit in the method file. With LSP enabled, the sample will be loaded using the intended loading flow. The sequence will be stopped immediately if the system gets partially blocked and the loading pressure limit is reached.

Q: Why should I use a venting T?

A venting tee speeds up loading significantly and prevents salts from being introduced into the MS.

Q: What can cause peak broadening?

The main reason for peak broadening is post-column mixing, primarily due to added swept volume.

Q: Can I use nanoViper tubing for an EASY-nLC II?

No.

Q: What should I do if I cannot establish network connection between EASY-nLC and Xcalibur via instrument configuration?

Verify that you are using the typical network benchtop setup for an EASY-nLC system connected to a Thermo Scientific MS - found in the User Guide, Chapter 2 “Installing an EASY-nLC Instrument”.
Verify that a network connection has been setup accordingly to the User Guide, Chapter 4 “Configuring the EASY-nLC system” sub section “setting up the network connection”.
Verify that there are no IP conflicts between the EASY-nLC IP address and other equipment.

Q: Why does my sequence in Xcalibur not start even though all devices are ready?

Normally a descriptive error message is shown if the sequence does not start, check for this message
Check that all necessary information with correct syntax is filled in the sequence list
Make sure the Sequence is not Paused
Power cycle the EASY-nLC, restart Xcalibur and check the connection in “instrument configuration”

Q: After upgrading my EASY-nLC software, why is the contact closure connection to the MS not working?

An EASY-nLC software upgrade will reset the instrument's contact closure settings.Always set up contact closure after a software upgrade. More information can be found in the User Guide, Chapter 4 “Configuring the EASY-nLC System"

Q: Why are the peptides eluting too late?

Increased retention time is typically caused by a leak or added swept volume of the system:
Run the system leak test
Verify that appropriate tubing dimensions are being used
Check that newly made connections are done properly and are not giving added swept volume
Check that Easy-nLC solvents are at the correct composition

Q: Why does Pump B give an error message that it is not able to reach target pressure?

The problem is normally caused by a leak between B pump and B valve, to diagnose run the leak test for the B pump. During the prepare gradient step of the method the B pump will try to reach the calculated gradient starting pressure (target pressure), if the pump does not reach the pressure it will abort the run and give the above error message.

Q: What is the min/max volume for autosampler custom wash?

The minimum wash volume for an autosampler custom wash is 0 µL, i.e. there is no lower limit
The maximum wash volume for an autosampler custom wash is loop volume + 8 µL
Example: for a 20 µL loop the max wash volume is 28 µL

Q: Where can I find a spare parts list?
Q: Where can I find release notes for new software versions for the EASY-nLC?

Release notes and latest software versions can be found EASY-nLC user support zone below

Q: What are the delay volumes of the EASY-nLC II and EASY-nLC 1000?

For EASY-nLC II the volume from gradient mixing tee to exit of column out tubing is 588 nL

For EASY-nLC 1000 the volume from gradient mixing tee to exit of column out tubing is 518 nL

Q: What backpressure/flow should I expect during pre-column and analytical column equilibration?

For an EASY-column (2cm, ID 100µm, 5µm particles), pre column equilibration @ 280 bar will typically give flow rates around 8-12 uL/min.
For an EASY-column (10cm, ID 75µm, 3µm particles), analytical column equilibration @ 280 bar will typically give flow rates around 800-1000 nL/min.
For a Pepmap column (2cm, ID 75µm, 3µm particles), pre column equilibration @ 800 bar will typically give flow rates around 7-9 uL/min.
For a Pepmap column (15cm, ID 50µm, 2µm particles), analytical column equilibration @ 800 bar will typically give flow rates around 400-500 nL/min.

Q: What are typical flush air volumes?

For the EASY-nLC II flush air volumes ≤ 10µL ensure good performance, but the lower the better

For the EASY-nLC 1000 flush air volumes ≤ 12µL ensure good performance, but the lower the better

Q: Why can I not connect my EASY-nLC to the remote support server?

Problems connecting to the remote support are typically caused by network firewalls. In order for an EASY-nLC to use remote support the local network firewall(s) must allow outgoing TCP/IP traffic from the EASY-nLC to the remote support server. Please consult your local IT administrator.

Q: Can I run the EASY-nLC maintenance scripts from Xcalibur?

No. The maintenance scripts have to be run directly from the EASY-nLC. Running maintenance scripts often require the user to take action by observing the system or connecting column out to waste in.

Q: Can I run the EASY-nLC from a remote PC?

No. Running the EASY-nLC from a remote computer is not supported

Q: Can I transfer method files from one EASY-nLC to another via USB key?

No, this is currently not possible. As an alternative, you can copy methods over the network via a PC.

EASY-nLC user support zone

This support zone is restricted to owners of EASY-nLC systems. Please enter your product key (the serial number of your instrument) to access. 

Service options

EASY-nLC systems have established a reputation for reliability and ease of use. However, ensuring the highest quality performance at nanoscale requires regular system checks and maintenance.

A range of support plans assist in proactively controlling service costs and improving productivity.

  • Critical Support Plan - the most comprehensive and personal support coverage for labs requiring high sample throughput and maximum uptime

  • Essential Support Plan - the most popular blend of support for labs seeking the ideal balance between cost control and instrument efficiency

  • Limited Support Plan - the most economical support coverage for labs seeking to maximize resources and instrument performance on a limited budget

  • Proactive Support Plan - the most complementary support coverage, offering affordable planned maintenance services to supplement internal service resources

Support plan range in detail

Four Levels of Coverage to Fit Your Needs
Enlarge
Contact Service and Support