For more information, click here.
The latest driver is included in LC devices, which can be ordered. Contact your local sales office for more info.
The latest driver can be found on the EASY-nLC User Zone - see below.
Load speed protection is designed to ensure that each sample is loaded with identical flow rates in order to maximize sample to sample reproducibility for quantitation experiments. LSP is only active during sample loading and for proper use of the LSP feature it is required to enter both a loading flow and a loading max pressure limit in the method file. With LSP enabled, the sample will be loaded using the intended loading flow. The sequence will be stopped immediately if the system gets partially blocked and the loading pressure limit is reached.
A venting tee speeds up loading significantly and prevents salts from being introduced into the MS.
The main reason for peak broadening is post-column mixing, primarily due to added swept volume.
Verify that you are using the typical network benchtop setup for an EASY-nLC system connected to a Thermo Scientific MS - found in the User Guide, Chapter 2 “Installing an EASY-nLC Instrument”.
Normally a descriptive error message is shown if the sequence does not start, check for this message
An EASY-nLC software upgrade will reset the instrument's contact closure settings.Always set up contact closure after a software upgrade. More information can be found in the User Guide, Chapter 4 “Configuring the EASY-nLC System"
Increased retention time is typically caused by a leak or added swept volume of the system:
The problem is normally caused by a leak between B pump and B valve, to diagnose run the leak test for the B pump. During the prepare gradient step of the method the B pump will try to reach the calculated gradient starting pressure (target pressure), if the pump does not reach the pressure it will abort the run and give the above error message.
The minimum wash volume for an autosampler custom wash is 0 µL, i.e. there is no lower limit
Spare parts for the EASY-nLC II can be found here: http://www.proxeon.com/productrange/nano_LC/accessories-spares/index.html
Release notes and latest software versions can be found EASY-nLC user support zone below
For EASY-nLC II the volume from gradient mixing tee to exit of column out tubing is 588 nL
For EASY-nLC 1000 the volume from gradient mixing tee to exit of column out tubing is 518 nL
For an EASY-column (2cm, ID 100µm, 5µm particles), pre column equilibration @ 280 bar will typically give flow rates around 8-12 uL/min.
For the EASY-nLC II flush air volumes ≤ 10µL ensure good performance, but the lower the better
For the EASY-nLC 1000 flush air volumes ≤ 12µL ensure good performance, but the lower the better
Problems connecting to the remote support are typically caused by network firewalls. In order for an EASY-nLC to use remote support the local network firewall(s) must allow outgoing TCP/IP traffic from the EASY-nLC to the remote support server. Please consult your local IT administrator.
No. The maintenance scripts have to be run directly from the EASY-nLC. Running maintenance scripts often require the user to take action by observing the system or connecting column out to waste in.
No. Running the EASY-nLC from a remote computer is not supported
No, this is currently not possible. As an alternative, you can copy methods over the network via a PC.
This support zone is restricted to owners of EASY-nLC systems. Please enter your product key (the serial number of your instrument) to access.
EASY-nLC systems have established a reputation for reliability and ease of use. However, ensuring the highest quality performance at nanoscale requires regular system checks and maintenance.
A range of support plans assist in proactively controlling service costs and improving productivity.