Nano-LC- EASY-nLC 1000
“Using the EASY-nLC 1000 system has increased our throughput by 25%”
Professor Matthias Mann, Max Planck Institute for Signal Transduction, Germany

Designed for reproducibility, reliability and productivity

  • Retention time variation routinely <0.4% RSD
  • Separation efficiency retained over shorter gradients

Superior separation and sharp peaks (FWHM 2-3 sec) in shorter gradients.

superior separations
With Thermo Scientific LTQ Velos and Nanospray Flex Ion Source
Sample:
Column:
Gradient:
Tryptic digest BSA
10 cm, 50 μm ID,1.9 μm C18 ReproSil AQ
5 – 35% ACN over 10 min
Short gradients demonstrate RT RSD (0.2%) - sharp peaks (2.1s) and high column peak capacity.

Efficient peptide analysis and protein identification in complex samples

efficient peptide analysis

With Thermo Scientific Orbitrap Velos and Proxeon ES070 nano-ES source

System:
Sample:
Gradient:
EASY-nLC 1000
HeLa cell lysates
240 min 5%–40%
Typical retention time deviation of key peaks <40 sec = RT RSD ~0.3%.

Trouble-free operation with dual in-line nano-flow sensors, pulsation-free pumps and innovative software control

  • Runs up to 384 samples unattended - refrigerated autosampler maintains sample stability
  • Uses ‘intelligent flow control’ to run according to user-defined pressure
    • Ensures rapid build-up of pressure and flow
    • Speeds up equilibration and sample loading
    • Continues analysis despite pressure fluctuations
  • Automated self-diagnostics indicate leak positions and detect flow-line blockages

2D-enabled operation

Identify more proteins

2D experiments identified 3-4 times as many proteins as 1D control

2D experiments

Venn diagram of identified number of proteins from analyses using the EASY-nLC system 2D configuration, conventional 2D (quaternary split-flow solvent delivery) or a 1D configuration.

Download technical note  'Identify more proteins compared to conventional approaches'  for more details