Applications and posters

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Efficient 2D LC-MS protein identification

Technical note: Identify more proteins compared to conventional approaches (331.6 kB)

Top-Down Protein Sequencing Using Nano-LCMS with Sequential Ion-Ion Reaction in a Dual- Pressure Linear Ion Trap

Andreas F.R. Hühmer1, Zhiqi Hao1, Shukun Luo2, Mengqiu Dong2 and David Horn1

1Thermo Fisher Scientific, San Jose, CA, USA; 2 National Institute of Biological Sciences, Beijing, China

Poster: Top-Down Protein Sequencing Using Nano-LCMS (530.1 kB)

Detection of Age-Related Histone PTMs as m/z Pair Tags in LC-MS Measurement of Mesenchymal Stem Cell Lysates

Michael Athanas1, D Sarracino2, B Krastins2, A Prakash2, T Rezai2, J Sutton2, V Lunyak3, M Dobke3, M F Lopez2

 1VAST Scientific, Cambridge, MA; 2Thermo Fisher Scientific BRIMS Center, Cambridge, MA; 3Buck Institute for Age Research, Novato, CA

Poster: Detection of Age-Related Histone PTMs (1.2 MB)

Splitless nano-LC for biomolecular research: proteomics to fossilomics (ABRF 2008)

Lisa M Freimark2; Alexandre Podtelej1; Michael B Andersen1; John M Lindsay1; Wenxia Zheng3; Mary H Schweitzer3; Brendan Manning4; Jeffrey Engelman5 and John M Asara2,6 

1Proxeon, Odense, Denmark,
2Division of Signal Transduction, Beth Israel Deaconess Medical Center, Boston,

3Department of Marine, Earth and Atmospheric Studies, North Carolina State University, Releigh,
Department of Genetics and Complex Diseases, Harvard Medical School, Boston, 

5Center for Thoracic Cancers, Massachusetts General Hospital, Boston,
Department of Pathology, Harvard Medical School, Boston.

Poster: Splitless nano-LC (1.8 MB)


PNAS  November 10, 2009 vol. 106 no. 45

Quantitative proteomic analysis of single pancreatic islets

Leonie F. Waanders a, Karolina Chwalek b, Mara Monettia, Chanchal Kumar a, Eckhard Lammert b,c, and Matthias Mann a,

a Department for Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany; b Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany; and c Institute of Metabolic Physiology, Heinrich Heine University, Universitatsstrasse 1, 40225 Dusseldorf, Germany

Science. 2009 May 1;324(5927):626-31

Biomolecular characterization and protein sequences of the Campanian hadrosaur B. canadensis.

Schweitzer MH, Zheng W, Organ CL, Avci R, Suo Z, Freimark LM, Lebleu VS, Duncan MB, Vander Heiden MG, Neveu JM, Lane WS, Cottrell JS, Horner JR, Cantley LC, Kalluri R, Asara JM.

Journal of Proteome Research, ACS, DOI: 10.1021, 2009


Automated 2D Peptide Separation on a 1D Nano-LC-MS System

Paul Taylor, Peter A. Nielsen, Morten B. Trelle, Ole B. Hørning, Michael B. Andersen, Ole Vorm, Michael F. Moran, and Thomas Kislinger

Journal of the American Society for Mass Spectrometry, September 2008

QCAL- a Novel Standard for Assessing Instrument Conditions for Proteome Analysis

Claire E. Eyers, Deborah M. Simpson, Stephen C.C. Wong, Robert J. Beynon and Simon J. Gaskell

Analytical Chemistry, April 2008

Performance of Combinatorial Peptide Libraries in Capturing the Low-Abundance Proteome of Red Blood Cells. 1. Behavior of Mono- to Hexapeptides.

Simó C,  Bachi A,  Cattaneo A,  Guerrier L, Fortis F., Boschetti E, Podtelejnikov A, and Righetti PG.

Analytical Chemistry, April 2008

Performance of combinatorial peptide libraries in capturing the low-abundance proteome. II: behaviour of resins containing individual amino acids.

Bachi  A., Simo C. Restuccia U., Guerrier L, Fortis F., Boschetti E., Masseroli M.d Righetti PG.

Neuroscience, February 2008

Decreased expression of the mitochondrial matrix proteases Lon and ClpP in cells from a patient with hereditary spastic paraplegia (SPG13)

Hansen J, Corydon TJ, Palmfeldt J, Dürr A, Fontaine B, Nielsen MN, Christensen JH, Gregersen N, Bross P.

Proteomics 2007. 7, 1575-1583

The phosphorylation pattern of bovine heart complex I subunits

Palmisano G., 2, Sardanelli A., Signorile A., Papa S., Larsen M.

Molecular and Cellular Proteomics 6 (10): 1711-1726 (2007)


Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis.

Niittylä T, Fuglsang AT, Palmgren MG, Frommer WB, Schulze WX

Plant Methods 2 (14): 1-11 (2006)

Metabolic labeling of plant cell cultures with K15NO3 as a tool for quantitative analysis of proteins and metabolites.

Engelsberger WR, Erban A, Kopka J, Schulze WX

Journal of Separation Science 29 (18): 2793-801 (2006)

Stable isotope-free quantitative shotgun proteomics combined with sample pattern recognition for rapid diagnostics.

Wienkoop S, Larrainzar E, Niemann M, Gonzalez EM, Lehmann U, Weckwerth W.